Journal: Lipids in Health and Disease

Article Title: C-reactive protein/oxidised low-density lipoprotein/β2-glycoprotein I complex promotes atherosclerosis in diabetic BALB/c mice via p38mitogen-activated protein kinase signal pathway

doi: 10.1186/1476-511X-12-42

Figure Lengend Snippet: Effect of CRP / oxLDL / β2 - GPI on infiltration of inflammatory cells in the aortas. Aortas were removed, paraffin-embedded, deparaffinated, underwent antigen retrieval, blocked, and then incubated with primary antibodies (Mac 1:200 or α- SMA 1:100 or CD3 1:100) at 4°C overnight. Biotinylated secondary antibody (1:200) was added, followed by HRP-labeled streptavidin incubation. Infiltrations of macrophages, SMCs and T cells in the aortas were quantified as the percentage of the luminal surface covered by positive cells to the plaque area using Image Pro-Plus 6.0. Results are shown as means ± SEM. A : Infiltration of SMCs in the aortas, 400× magnification. B : Infiltration of macrophages in the aortas, 400× magnification. C : Infiltration of T cells in aortas, 400× magnification. D : Positive expressions of SMA, MAC and CD3 (n=4), expressed as the percentage to plaque area. * P <0.05, compared with the CRP/oxLDL/β2GPI group, † P < 0.05, compared with the PBS group.

Article Snippet: Mice anti-α-smooth muscle actin (SMA, BM0002) antibody, rabbit anti-CD3 (BA0429) antibody were purchased from Boster, Wuhan, China.

Techniques: Incubation, Labeling